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Effect of Temperature on Heart Rate for <i>Phaenicia sericata</i> and <i>Drosophila melanogaster</i> with Altered Expression of the TrpA1 Receptors.

Author
Abstract
:

The transient receptor potential (TrpA-ankyrin) receptor has been linked to pathological conditions in cardiac function in mammals. To better understand the function of the TrpA1 in regulation of the heart, a model was used to express TrpA1 in heart and body wall muscles. Heartbeat of in intact larvae as well as hearts in situ, devoid of hormonal and neural input, indicate that strong over-expression of TrpA1 in larvae at 30 or 37 °C stopped the heart from beating, but in a diastolic state. Cardiac function recovered upon cooling after short exposure to high temperature. Parental control larvae (UAS-TrpA1) increased heart rate transiently at 30 and 37 °C but slowed at 37 °C within 3 min for in-situ preparations, while in-vivo larvae maintained a constant heart rate. The in-situ preparations maintained an elevated rate at 30 °C. The heartbeat in the TrpA1-expressing strains could not be revived at 37 °C with serotonin. Thus, TrpA1 activation may have allowed enough Ca influx to activate K() channels into a form of diastolic stasis. TrpA1 activation in body wall muscle confirmed a depolarization of membrane. In contrast, blowfly larvae increased heartbeat at 30 and 37 °C, demonstrating greater cardiac thermotolerance.

Year of Publication
:
2021
Journal
:
Insects
Volume
:
12
Issue
:
1
Date Published
:
2021
URL
:
https://www.mdpi.com/resolver?pii=insects12010038
DOI
:
10.3390/insects12010038
Short Title
:
Insects
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