Preliminary report on fractionation of fucans by ion-exchange displacement centrifugal partition chromatography.
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Abstract |
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A new method combining ion-exchange displacement chromatography with centrifugal partition chromatography (CPC) was used for the fractionation of partially depolymerized fucans (polysulphated polysaccharides). The ion-exchanger was Amberlite LA2, a high-molecular-mass liquid secondary amine miscible with most common organic solvents and immiscible with aqueous solutions. Ion-exchange displacement centrifugal partition chromatography was performed with LA2 in methyl isobutyl ketone (MiBK) as the stationary phase, water as the mobile phase, Cl- as the carrier and OH- as the displacer. A complex mixture of partially depolymerized fucans was resolved into adjacent families characterized by their peak molecular mass and polydispersity. The Dubois test (sugar) and the azur A test (SO3-) confirmed the displacement mode of the process, and size-exclusion chromatographic controls confirmed its efficiency. |
Year of Publication |
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1998
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Journal |
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Journal of chromatography. B, Biomedical sciences and applications
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Volume |
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706
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Issue |
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1
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Number of Pages |
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43-54
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Date Published |
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1998
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ISSN Number |
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1387-2273
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DOI |
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10.1016/s0378-4347(97)00448-9
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Short Title |
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J Chromatogr B Biomed Sci Appl
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